Recently, the school of medicine, Qiingdao University School of Medicine researchers published a paper in order to understand the protective effect and mechanism of scallop polypeptide on H2O2 induced oxidative damage in HaCaT cells. The results showed that the polypeptide from 1.42 to 5.68mmol/L could eliminate the ROS in the cell and inhibit the expression of Gadd45a protein, which could protect H2O2 cells from oxidative stress induced by HaCaT. This article is published in the 2014 issue of the 04 issue of the Journal of Qiingdao University medical college.

Logarithmic growth phase HaCaT cells were randomly divided into normal control group (normal cell culture), model group (H2O2 12h 200 mol/L), Scallop in Shell polypeptides with different concentration groups (low, medium and high concentrations, respectively 1.42, 2.84, 5.68mmol/L Scallop in Shell peptide pretreatment after 2H to 200 mol/L H2O2 12h), Hochest33258 staining was used to detect the apoptotic rate, the survival rate of CCK8 cells was detected by fluorescent yellow, two hydrogen diacetate (DCFH-DA) as active oxygen fluorescent probe and flow cytometry in cell (ROS) content, protein blot detection of growth arrest and DNA damage induced by repair gene 45A (Gadd45a protein) expression.

Compared with the normal control group, the apoptosis rate of model group increased significantly (F=439.80, q=38.24, P<0.01), the cell survival rate decreased (F=191.40, q=214.10, P<0.01), increase the content of intracellular ROS (F=121.10, q=31.02, P<0.01), Gadd45a protein expression increased (F=66.59, q=14.57, P<0.01); compared with the model group. Scallop in Shell peptide groups apoptosis rate was significantly lower (q=9.60 ~ 25.82, P<0.01), cell survival rate increased (q=13.47 ~ 54.86, P<0.01), decreasing intracellular ROS (q=4.20 ~ 12.14, P<0.01), lower Gadd45a protein expression (q=4.04 ~ 15.55, P<0.01); Scallop in Shell peptide in different concentration groups compared with Scallop in Shell peptide concentration, apoptosis rate, cell survival rate increased, decreased the content of ROS cells decreased the expression of Gadd45a, there were significant differences (q=3.96 ~ 23.36, P<0.05).